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蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析   总被引:4,自引:0,他引:4  
利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌(Coelomactra antiquata)、中国蛤蜊(Mactra chinensis)和四角蛤蜊(Mactra veneriformis)3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列.测序后用DNAstar软件分析了核苷酸差异。结果显示:三种贝类16SrRNA基因片段长度相同,均为306bp(去除引物).核苷酸存在多态性。共有45个变异位点,54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88.9%.与四角蛤蜊的同源性为88.6%.中国蛤蜊与四角蛤蜊的同源性为90.6%。三种蛤蜊ITS2序列分别为390bp(西施舌)、441bp(四角蛤蜊)和466bp(中国蛤蜊)。存在长度多态性.ITS2核苷酸差异分析结果显示.西施舌与中国蛤蜊的同源性为70.9%-71.1%,西施舌与四角蛤蜊的为70.5%-71.0%。中国蛤蜊与四角蛤蜊的同源性为88.1%-88.8%。ITS2序列分析结果与16SrRNA基因片段分析结果一致.2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。  相似文献   
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We consider sulphur depletion in dense molecular clouds, and suggest hydrated sulphuric acid, H2SO4 ·  n H2O, as a component of interstellar dust in icy mantles. We discuss the formation of hydrated sulphuric acid in collapsing clouds and its instability in heated regions in terms of the existing hot core models and observations. We also show that some features of the infrared spectrum of hydrated sulphuric acid have correspondence in the observed spectra of young stellar objects.  相似文献   
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We present an analysis of X-ray and ultraviolet (UV) data of the dwarf nova VW Hyi that were obtained with XMM–Newton during the quiescent state. The X-ray spectrum indicates the presence of an optically thin plasma in the boundary layer that cools as it settles on to the white dwarf. The plasma has a continuous temperature distribution that is well described by a power law or a cooling flow model with a maximum temperature of 6–8 keV. We estimate from the X-ray spectrum a boundary layer luminosity of  8 × 1030 erg s-1  , which is only 20 per cent of the disc luminosity. The rate of accretion on to the white dwarf is  5 × 10−12 M yr−1  , about half of the rate in the disc. From the high-resolution X-ray spectra, we estimate that the X-ray emitting part of the boundary layer is rotating with a velocity of 540 km s−1, which is close to the rotation velocity of the white dwarf but is significantly smaller than the Keplerian velocity. We detect a 60-s quasi-periodic oscillation of the X-ray flux, which is likely to be due to the rotation of the boundary layer. The X-ray and the UV flux show strong variability on a time-scale of ∼1500 s. We find that the variability in the two bands is correlated and that the X-ray fluctuations are delayed by ∼100 s. The correlation indicates that the variable UV flux is emitted near the transition region between the disc and the boundary layer and that accretion rate fluctuations in this region are propagated to the X-ray emitting part of the boundary layer within ∼100 s. An orbital modulation of the X-ray flux suggests that the inner accretion disc is tilted with respect to the orbital plane. The elemental abundances in the boundary layer are close to their solar values.  相似文献   
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We present a collation of the available data on the opening angles of jets in X-ray binaries, which in most cases are small (≲10°). Under the assumption of no confinement, we calculate the Lorentz factors required to produce such small opening angles via the transverse relativistic Doppler effect. The derived Lorentz factors, which are in most cases lower limits, are found to be large, with a mean >10, comparable to those estimated for active galactic nuclei (AGN) and much higher than the commonly assumed values for X-ray binaries of 2–5. Jet power constraints do not, in most cases, rule out such high Lorentz factors. The upper limits on the opening angles show no evidence for smaller Lorentz factors in the steady jets of Cygnus X-1 and GRS 1915+105. In those sources in which deceleration has been observed (notably  XTE J1550−564  and Cygnus X-3), some confinement of the jets must be occurring, and we briefly discuss possible confinement mechanisms. It is however possible that all the jets could be confined, in which case the requirement for high bulk Lorentz factors can be relaxed.  相似文献   
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